1ZC0
Crystal structure of human hematopoietic tyrosine phosphatase (HePTP) catalytic domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-31 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9797, 0.9791, 0.8856 |
Spacegroup name | P 61 |
Unit cell lengths | 127.101, 127.101, 59.863 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.850 |
R-factor | 0.16257 |
Rwork | 0.161 |
R-free | 0.18584 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.329 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.080 | |
Number of reflections | 47311 | |
Completeness [%] | 100 | |
Redundancy | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 277 | Sodium/potassium phosphate, acetate, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |