1ZBS
Crystal Structure of the Putative N-acetylglucosamine Kinase (PG1100) from Porphyromonas gingivalis, Northeast Structural Genomics Target PgR18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.506, 89.506, 82.323 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.300 - 2.300 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.28900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.120 | 0.500 |
| Number of reflections | 32892 | |
| <I/σ(I)> | 19.4 | 4.1 |
| Completeness [%] | 99.6 | 96.9 |
| Redundancy | 11.8 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 50 mM (Na)3-citrate (pH 4), 16% PEG1K, 100 mM NH4Br, and 5 mM DTT., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
