1ZBR
Crystal Structure of the Putative Arginine Deiminase from Porphyromonas gingivalis, Northeast Structural Genomics Target PgR3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97928 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.824, 86.049, 144.641 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.900 - 2.600 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.28900 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.097 | 0.331 |
Number of reflections | 37449 | |
<I/σ(I)> | 161.5 | 5.97 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.3 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES, 10% isopropanol, 20% PEG4K, 5 DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |