1ZBM
X-Ray Crystal Structure of Protein AF1704 from Archaeoglobus fulgidus. Northeast Structural Genomics Consortium Target GR62A.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97912 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.784, 69.931, 105.581 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.310 - 2.300 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.26500 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.089 | 0.252 |
Number of reflections | 24762 | |
<I/σ(I)> | 20.07 | 8.01 |
Completeness [%] | 99.8 | |
Redundancy | 6.1 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM sodium acetate, 20% PEG4k, 200mM ammonium acetate, 5mM DTT., pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |