1ZBC
Crystal Structure of the porcine signalling protein liganded with the peptide Trp-Pro-Trp (WPW) at 2.3 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.350, 66.480, 107.026 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.290 |
| R-factor | 0.19822 |
| Rwork | 0.193 |
| R-free | 0.23479 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xrv |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.325 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 19223 | |
| <I/σ(I)> | 10 | 2.1 |
| Completeness [%] | 99.3 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 299 | 25mM Tris-HCl, 14% ethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 299K |
