1ZB1

Structure basis for endosomal targeting by the Bro1 domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Wavelength(s)	0.9796,0.9790,0.9720
Spacegroup name	P 1 21 1
Unit cell lengths	76.445, 58.274, 120.823
Unit cell angles	90.00, 90.79, 90.00

Refinement procedure

Resolution	40.260 - 1.950
Rwork	0.224
R-free	0.25200
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.300	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	77077
Completeness [%]		95.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	298	PEG 3350, tri-potassium citrate, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K