1ZAT
Crystal Structure of an Enterococcus faecium peptidoglycan binding protein at 2.4 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979676 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 115.976, 115.976, 68.275 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.920 - 2.400 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.25700 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.920 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 20893 | |
<I/σ(I)> | 6.4 | 1.5 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 8 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 291 | ammonium sulfate, sodium chloride, sodium acetate, PEG 2000, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |