1ZAP
SECRETED ASPARTIC PROTEASE FROM C. ALBICANS
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1993-06 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.600, 98.260, 49.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.145 |
| Rwork | 0.145 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 26.780 * |
| Data reduction software | DENZO ((HKL)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.099 |
| Total number of observations | 30743 * |
| Number of reflections | 10193 |
| Completeness [%] | 89.0 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5-10 (mg/ml) | |
| 2 | 1 | 1 | glycine | 25 (mM) | |
| 3 | 1 | 1 | 25 (mM) | ||
| 4 | 1 | 2 | imidazole/malate | 40 (mM) | |
| 5 | 1 | 2 | 1 (mM) | ||
| 6 | 1 | 2 | PEG8000 | 22-24 (%) |
