1ZAO
Crystal Structure of A.fulgidus Rio2 Kinase Complexed With ATP and Manganese Ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-08 |
Detector | MARRESEARCH |
Wavelength(s) | 0.99997 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.862, 44.365, 62.793 |
Unit cell angles | 90.00, 94.01, 90.00 |
Refinement procedure
Resolution | 63.250 - 1.840 |
R-factor | 0.1835 |
Rwork | 0.183 |
R-free | 0.21240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tqi |
RMSD bond length | 0.014 |
RMSD bond angle | 1.414 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.250 | 1.887 |
High resolution limit [Å] | 1.840 | 1.840 |
Number of reflections | 27131 | |
Completeness [%] | 68.2 | |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.78 | 293 | PEG 900, SODIUM PHOSPHATE, SODIUM CITRATE , pH 3.78, VAPOR DIFFUSION, HANGING DROP, temperature 293K |