1ZAK
ADENYLATE KINASE FROM MAIZE IN COMPLEX WITH THE INHIBITOR P1,P5-BIS(ADENOSINE-5'-)PENTAPHOSPHATE (AP5A)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-04 |
| Detector | SIEMENS-NICOLET X100 |
| Spacegroup name | P 3 |
| Unit cell lengths | 83.300, 83.300, 69.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.500 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ake |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.000 * |
| Data reduction software | XDS |
| Data scaling software | MERGE |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.330 |
| High resolution limit [Å] | 3.160 | 3.160 |
| Rmerge | 0.074 * | |
| Number of reflections | 6998 | |
| Completeness [%] | 75.4 | 53.3 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 7 (mg/ml) | |
| 2 | 1 | drop | Ap5A | 2 (mM) | |
| 3 | 1 | drop | HEPES | 10 (mM) | |
| 4 | 1 | drop | 1 (M) | ||
| 5 | 1 | drop | PEG8000 | 10 (%) | |
| 6 | 1 | reservoir | 1 (M) | ||
| 7 | 1 | reservoir | PEG8000 | 10 (%) |
