1ZAH
Fructose-1,6-bisphosphate aldolase from rabbit muscle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.096, 102.916, 84.663 |
Unit cell angles | 90.00, 98.36, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
Rwork | 0.167 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ado |
RMSD bond length | 0.005 |
RMSD bond angle | 1.267 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.049 | 0.388 |
Number of reflections | 127047 | |
Completeness [%] | 97.0 | 87.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | sodium HEPES, PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |