1ZAG
HUMAN ZINC-ALPHA-2-GLYCOPROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.600, 131.700, 118.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| Rwork | 0.229 |
| R-free | 0.28800 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.066 | 0.453 |
| Number of reflections | 39365 | |
| <I/σ(I)> | 27 | 2.5 |
| Completeness [%] | 95.6 | 75.7 |
| Redundancy | 4.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | Sanchez, L.M., (1997) Proc.Nat.Acad.Sci.USA, 94, 4626. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 0.02 (%) | ||
| 4 | 1 | reservoir | mPEG2000 | 30 (%(w/v)) | |
| 5 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) |
