1ZAB
Crystal Structure of Mouse Cytidine Deaminase Complexed with 3-Deazauridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-23 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 82.237, 92.361, 180.531 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.560 - 2.360 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fr5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | BBS |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.560 | 2.440 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.095 | 0.290 |
| Number of reflections | 28544 | |
| <I/σ(I)> | 9.8 | 4.4 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 5.21 | 5.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | Ammonium sulphate, citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
