1ZA6
The structure of an antitumor CH2-domain-deleted humanized antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-04 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 82.540, 224.200, 166.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.130 - 2.800 |
R-factor | 0.246 |
Rwork | 0.246 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1BBJ; PDB ENTRY 1FC1 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.880 |
Data reduction software | d*TREK (V. 9.0) |
Data scaling software | d*TREK (V. 9.0) |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.130 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.152 | 0.365 |
Number of reflections | 76662 | |
<I/σ(I)> | 10.9 | 5 |
Completeness [%] | 99.9 | 98 |
Redundancy | 9.06 | 7.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 290 | 4 M sodium formate, 1.5 mM Triton X-100 detergent, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 290K, pH 7.20 |