1Z9T
CRYSTAL STRUCTURE OF A PUTATIVE LACCASE (YFIH) FROM ESCHERICHIA COLI AT 1.54 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-16 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.711, 80.711, 90.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.300 - 1.540 |
| R-factor | 0.14324 |
| Rwork | 0.142 |
| R-free | 0.17200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u05 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.732 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.300 | 60.300 | 1.580 |
| High resolution limit [Å] | 1.540 | 6.890 | 1.540 |
| Number of reflections | 44917 | ||
| <I/σ(I)> | 6.7 | 18 | 1 |
| Completeness [%] | 100.0 | ||
| Redundancy | 6.8 | 5.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 5 | 277 | 0.2M (NH4)2HCitrate, 20.0% PEG-3350, No Buffer pH 5.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
