1Z9P
X-Ray structure of a Cu-Zn superoxide dismutase from Haemophilus ducreyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.410, 63.990, 73.970 |
| Unit cell angles | 90.00, 118.04, 90.00 |
Refinement procedure
| Resolution | 19.320 - 1.500 |
| R-factor | 0.17228 |
| Rwork | 0.170 |
| R-free | 0.21238 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z9n |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.539 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.178 |
| Number of reflections | 46454 | |
| <I/σ(I)> | 17.99 | 6.62 |
| Completeness [%] | 97.4 | 91.4 |
| Redundancy | 3.93 | 3.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | sodium cytrate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
