1Z9L
1.7 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795, 0.9793, 0.9649 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 48.200, 48.200, 112.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.580 - 1.700 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.25400 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 27860 | |
Completeness [%] | 98.8 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 283 | PEG2000 MME, NaCl, tris buffer, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K |