1Z9K

Photosynthetic Reaction Center from Rhodobacter sphaeroides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2003-08-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 42 2 2
Unit cell lengths	207.800, 207.800, 107.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 4.600
R-factor	0.34
Rwork	0.330
R-free	0.33000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m3x
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	4.850
High resolution limit [Å]	4.600	4.600
Rmerge	0.182
Number of reflections	12646
<I/σ(I)>	5.3
Completeness [%]	94.2	91
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	PEG, NaCL, MnCl, LDAO, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 298K