1Z9J
Photosynthetic Reaction Center from Rhodobacter sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 203.840, 203.840, 119.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 4.500 |
| R-factor | 0.299 |
| Rwork | 0.299 |
| R-free | 0.33800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m3x |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 4.800 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.190 | 0.860 |
| Number of reflections | 13804 | |
| <I/σ(I)> | 3.1 | |
| Completeness [%] | 87.7 | 92 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | PEG,NaCl,MnCl, LDAO, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
