1Z9H
Microsomal prostaglandin E synthase type-2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2004-08-10 |
| Wavelength(s) | 0.99108 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.240, 122.830, 111.530 |
| Unit cell angles | 90.00, 110.60, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.215 |
| Rwork | 0.214 |
| R-free | 0.25100 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 47852 | |
| Completeness [%] | 99.2 | 84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | Ammonia sulfate, sodium acetate, indomethacin, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
