1Z9G
Crystal Structure Analysis of Thermolysin Complexed with the Inhibitor (R)-retro-thiorphan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 298 |
| Detector technology | FILM |
| Collection date | 1987 |
| Detector | KODAK |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 94.000, 94.000, 132.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3TLN |
| RMSD bond length | 0.022 |
| RMSD bond angle | 3.250 |
| Data reduction software | ROTAVATA |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.058 |
| Number of reflections | 27574 |
| Completeness [%] | 72.0 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | dilution with H2O | 7.2 | 277 | Tris acetate, calcium acetate, DMSO, pH 7.2, dilution with H2O, temperature 277K |
