1Z9C

Crystal structure of OhrR bound to the ohrA promoter: Structure of MarR family protein with operator DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-01
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	81.899, 80.960, 109.340
Unit cell angles	90.00, 100.20, 90.00

Refinement procedure

Resolution	59.650 - 2.640
R-factor	0.216
Rwork	0.216
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	EPMR (2.5)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.650	2.760
High resolution limit [Å]	2.640	2.640
Rmerge	0.054	0.386
Number of reflections	47153
<I/σ(I)>	11.1	2
Completeness [%]	96.9	96.9
Redundancy	2.3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.7	298	pH 3.7, 0.1 M MgCl2 and 5 to 8 % of PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MgCl2
2	1	1	PEG 8000
3	1	2	MgCl2
4	1	2	PEG 8000