1Z96
Crystal structure of the Mud1 UBA domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-02 |
Detector | MARRESEARCH |
Wavelength(s) | 1.771 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 60.601, 60.601, 96.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.890 - 1.800 |
R-factor | 0.16801 |
Rwork | 0.167 |
R-free | 0.19593 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.221 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.868 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.050 | 0.444 |
Number of reflections | 10214 | |
<I/σ(I)> | 10.1 | 1.7 |
Completeness [%] | 99.8 | 99.79 |
Redundancy | 10.3 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | sodium formate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | sodium formate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |