1Z8X
Structure of Mutant Pyrrolidone Carboxyl Peptidase (E192V) from a Hyperthermophile, Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.547, 104.536, 77.363 |
| Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| Rwork | 0.220 |
| R-free | 0.24610 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1z8t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.041 | 0.069 |
| Number of reflections | 59076 | |
| <I/σ(I)> | 30.3 | 16.7 |
| Completeness [%] | 96.2 | 73.1 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
