1Z8N

Crystal structure of Arabidopsis thaliana Acetohydroxyacid synthase In Complex With An Imidazolinone Herbicide, Imazaquin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-12
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	P 64 2 2
Unit cell lengths	179.081, 179.081, 186.077
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.2004
Rwork	0.199
R-free	0.21570
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1ybh
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.060
Number of reflections	40076
<I/σ(I)>	23	3.26
Completeness [%]	90.8	56
Redundancy	5.7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	290	Tris, FAD, DTT, ThDP, magnesium chloride, imazaquin, CHES, lithium sufate, potassium sodium tartrate , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K