1Z8H
CRYSTAL STRUCTURE OF a GDSL-like lipase (ALR1529) FROM NOSTOC SP. PCC 7120 AT 2.02 A RESOLUTION
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9787, 1.0332,0.9784 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.504, 69.806, 211.028 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.550 - 2.020 |
R-factor | 0.1567 |
Rwork | 0.155 |
R-free | 0.19469 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.469 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.550 | 2.090 |
High resolution limit [Å] | 2.020 | 2.020 |
Number of reflections | 62663 | |
<I/σ(I)> | 26.76 | 4.9 |
Completeness [%] | 99.0 | 95.27 |
Redundancy | 9.6 | 7.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 10% NP_Isopropanol, 0.06M HEPES, 0.04M HEPES_Na, 12% PEG 4000, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |