1Z8D
Crystal Structure of Human Muscle Glycogen Phosphorylase a with AMP and Glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.370, 144.000, 59.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.790 - 2.300 |
Rwork | 0.192 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7gpb |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNX (2000.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.700 | 2.360 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.068 | 0.201 |
Number of reflections | 36521 | |
<I/σ(I)> | 11.4 | 5 |
Completeness [%] | 97.5 | 93.1 |
Redundancy | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 277 | PEG mme 550, glucose, TRIS, spermine, DTT, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |