1Z7C

Crystal Structure of Human Placental Lactogen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	93
Detector technology	CCD
Collection date	2001-06-03
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	115.504, 30.047, 53.490
Unit cell angles	90.00, 113.38, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.23308
Rwork	0.229
R-free	0.26704
Structure solution method	MAD
RMSD bond length	0.023
RMSD bond angle	2.141
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	RESOLVE (2.06)
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.900
High resolution limit [Å]	1.900	1.900
Rmerge	0.050	0.505
Number of reflections	13322
<I/σ(I)>	21.9	1.5
Completeness [%]	95.9	70.6
Redundancy	3.6	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	sodium phosphate, PEG 3400, sodium chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	sodium phosphate, PEG 3400, sodium chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	sodium phosphate, PEG 3400, sodium chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K