1Z7A
Crystal structure of probable Polysaccharide deacetylase from Pseudomonas aeruginosa PAO1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-20 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97945 |
Spacegroup name | P 1 |
Unit cell lengths | 101.653, 101.773, 100.990 |
Unit cell angles | 60.71, 61.24, 89.90 |
Refinement procedure
Resolution | 50.000 - 1.710 |
R-factor | 0.15224 |
Rwork | 0.151 |
R-free | 0.17461 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.213 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.082 | 0.303 |
Number of reflections | 304779 | |
<I/σ(I)> | 16.7 | 2.87 |
Completeness [%] | 71.2 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | PEG4K, i-PrOH, NaCitrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |