1Z78
Crystal Structure of the Thrombospondin-1 N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.07218 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.812, 41.899, 53.458 |
| Unit cell angles | 90.00, 100.65, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.237 |
| Rwork | 0.236 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NTSP-1 structure from NTSP-1/Arixtra complex to be deposited |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.630 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.068 | 0.427 |
| Number of reflections | 17415 | |
| <I/σ(I)> | 32.12 | 2.75 |
| Completeness [%] | 99.6 | 96.8 |
| Redundancy | 6.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 30% PEG1500 and 0.1 M NaAc, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
