1Z72
Structure of a putative transcriptional regulator from Streptococcus pneumoniae
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2005-02-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97908, 0.97919 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 116.023, 122.039, 46.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.100 - 1.450 |
R-factor | 0.15198 |
Rwork | 0.151 |
R-free | 0.16709 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.176 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.510 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.400 | |
Number of reflections | 116194 | |
<I/σ(I)> | 2.4 | |
Completeness [%] | 91.5 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |