1Z6U
Np95-like ring finger protein isoform b [Homo sapiens]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-25 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.561, 64.561, 130.313 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.860 - 2.100 |
| R-factor | 0.22507 |
| Rwork | 0.222 |
| R-free | 0.28274 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.315 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.860 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.568 |
| Number of reflections | 16546 | |
| <I/σ(I)> | 24.8 | 3.37 |
| Completeness [%] | 98.8 | 99.9 |
| Redundancy | 7.7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 298 | 0.8 M NA CITRATE, 0.1 M HEPES PH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 6.50 |
