1Z6T

Structure of the apoptotic protease-activating factor 1 bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-01
Detector	ADSC QUANTUM 4
Spacegroup name	P 1
Unit cell lengths	75.955, 92.883, 94.988
Unit cell angles	62.96, 89.99, 90.05

Refinement procedure

Resolution	15.000 - 2.210
R-factor	0.192
Rwork	0.189
R-free	0.24400
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.431
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.270
High resolution limit [Å]	2.200	2.210
Rmerge	0.048
Number of reflections	103300
<I/σ(I)>	12.7	2.5
Completeness [%]	89.5	48.9
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.1	276	HEPES, ammonium acetate, PEG-3350, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 276K, pH 7.10
1		7.1	276	HEPES, ammonium acetate, PEG-3350, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 276K, pH 7.10