1Z6R
Crystal structure of Mlc from Escherichia coli
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-05 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97637, 0.97866 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 235.950, 74.710, 154.950 |
Unit cell angles | 90.00, 129.15, 90.00 |
Refinement procedure
Resolution | 19.940 - 2.700 |
R-factor | 0.20564 |
Rwork | 0.203 |
R-free | 0.26265 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.548 |
Data scaling software | XDS |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 57176 | |
<I/σ(I)> | 12.2 | 2 |
Completeness [%] | 98.4 | 93.6 |
Redundancy | 7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.6M MgSO4, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |