1Z6K
Citrate lyase beta subunit complexed with oxaloacetate and magnesium from M. tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2004-11-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 91.280, 91.280, 219.263 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.300 |
R-factor | 0.256 |
Rwork | 0.224 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u5h |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 14893 |
Completeness [%] | 93.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 300 | sodium formate, DMSO., pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K |