1Z67
Structure of Homeodomain-like Protein of Unknown Function S4005 from Shigella flexneri
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-07 |
Detector | SBC-3 |
Wavelength(s) | 0.97934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 57.476, 40.479, 48.333 |
Unit cell angles | 90.00, 93.78, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.450 |
R-factor | 0.1643 |
Rwork | 0.162 |
R-free | 0.20790 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.427 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.040 | 0.330 |
Number of reflections | 17111 | |
<I/σ(I)> | 39.2 | 2.14 |
Completeness [%] | 86.5 | 26.5 |
Redundancy | 4.1 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Bis-Tris, 2 M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |