1Z41
Crystal structure of oxidized YqjM from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.510, 185.230, 169.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.300 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.20800 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.550 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 183799 | |
<I/σ(I)> | 20.6 | 2 |
Completeness [%] | 92.7 | 82.2 |
Redundancy | 2.35 | 2.09 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292.15 | Tris/HCl, PEG 3500, lithium sulfate, strontium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 292.15K |