1Z3X

Structure of Gun4 from Thermosynechococcus elongatus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS
Synchrotron site	NSLS
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.976, 0.9802, 0.9660
Spacegroup name	P 1 21 1
Unit cell lengths	58.295, 40.672, 62.372
Unit cell angles	90.00, 115.40, 90.00

Refinement procedure

Resolution	50.000 - 1.500
R-factor	0.18042
Rwork	0.179
R-free	0.20856
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.117
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.539
High resolution limit [Å]	1.500	1.500
Rmerge	0.068	0.467
Number of reflections	42482
Completeness [%]		100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% PEG 3350, 0.2 M NH3Cl, VAPOR DIFFUSION, SITTING DROP, temperature 277K