1Z3L

X-Ray Crystal Structure of a Mutant Ribonuclease S (F8Anb)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2003-12-05
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	44.464, 44.464, 97.469
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.970 - 1.800
R-factor	0.197
Rwork	0.197
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rln
RMSD bond length	0.004
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.071	0.208
Number of reflections	10679
Completeness [%]	97.8	97
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method	5.75	293	ammonium sulfate, cesium chloride, sodium acetate, pH 5.75, Batch method, temperature 293K