1Z2X
Crystal structure of mouse Vps29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.912, 68.321, 60.166 |
| Unit cell angles | 90.00, 106.98, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.220 |
| R-factor | 0.17591 |
| Rwork | 0.173 |
| R-free | 0.22765 |
| Structure solution method | MIR |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.829 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.069 | 0.216 |
| Number of reflections | 21420 | |
| <I/σ(I)> | 16.8 | 6.3 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M Tris, 0.2M NaCl, 20% PEG3000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
