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1Z2W

Crystal structure of mouse Vps29 complexed with Mn2+

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSRS BEAMLINE PX9.6
Synchrotron siteSRS
BeamlinePX9.6
Temperature [K]110
Detector technologyCCD
Collection date2004-06-11
DetectorADSC QUANTUM 4
Wavelength(s)0.87
Spacegroup nameP 1 21 1
Unit cell lengths55.914, 68.953, 60.519
Unit cell angles90.00, 106.65, 90.00
Refinement procedure
Resolution20.000 - 2.000
R-factor0.17939
Rwork0.176
R-free0.23742
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1z2x
RMSD bond length0.026
RMSD bond angle2.134
Data scaling softwareCCP4 ((SCALA))
Refinement softwareREFMAC (5.1.24)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]57.0002.100
High resolution limit [Å]2.0002.000
Rmerge0.0700.441
Number of reflections30024
<I/σ(I)>12.93.7
Completeness [%]97.697.6
Redundancy3.53.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8.52890.1M Tris, 0.2M NaCl, 20% PEG3000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K

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