1Z2I
CRYSTAL STRUCTURE OF Agrobacterium tumefaciens MALATE DEHYDROGENASE, NEW YORK STRUCTURAL GENOMICS CONSORTIUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 87 |
Detector technology | IMAGE PLATE |
Collection date | 2005-02-11 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 31 |
Unit cell lengths | 98.220, 98.220, 146.470 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.26500 |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.087 | 0.208 |
Number of reflections | 71329 | |
<I/σ(I)> | 8.9 | 2.6 |
Completeness [%] | 89.1 | 53.4 |
Redundancy | 15.9 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | PEG3350, 0.1M Bis-Tris, 0.1M Lithium sulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |