1Z2A
GDP-Bound Rab23 GTPase crystallized in P2(1)2(1)2(1) space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-30 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.281, 61.440, 63.854 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.2322 |
| Rwork | 0.230 |
| R-free | 0.27761 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Polyalanine Rab3a |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.085 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 12522 | |
| <I/σ(I)> | 24.5 | 5.3 |
| Completeness [%] | 95.9 | 79.4 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 15% PEG 6000, 50mM Na acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
