1Z21
Crystal structure of the core domain of Yersinia pestis virulence factor YopR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-15 |
| Detector | BRANDEIS |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 85.662, 85.662, 85.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.810 - 1.499 |
| R-factor | 0.20526 |
| Rwork | 0.204 |
| R-free | 0.23833 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.019 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.820 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.036 | |
| Number of reflections | 17807 | |
| <I/σ(I)> | 46.1 | |
| Completeness [%] | 99.3 | 97.5 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 291 | 17% PEG-8000, 85 mM Phosphate-citrate, 0.17 M NaCl, and 15% glycerol, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
