1Z1Y
Crystal structure of Methylated Pvs25, an ookinete protein from Plasmodium vivax
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.09524 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.164, 43.700, 65.650 |
Unit cell angles | 90.00, 103.15, 90.00 |
Refinement procedure
Resolution | 28.600 - 2.000 |
R-factor | 0.245 |
Rwork | 0.244 |
R-free | 0.27600 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.364 |
Data reduction software | HKL-2000 |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.600 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.083 | 0.187 |
Number of reflections | 21346 | |
<I/σ(I)> | 12.4 | 5.2 |
Completeness [%] | 98.7 | 98.1 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG1500, sodium accetate, ammonium accetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |