1Z1W
Crystal structures of the tricorn interacting facor F3 from Thermoplasma acidophilum, a zinc aminopeptidase in three different conformations
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-13 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9788, 0.9792, 0.9200 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 104.786, 104.786, 137.348 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.920 - 2.700 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.29500 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.920 | 2.890 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 24288 | |
Completeness [%] | 99.2 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | PEG 2000, Lithium sulphate, TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |