1Z1N
Crystal Structure of the sixteen heme cytochrome from Desulfovibrio gigas
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.738, 1.744, 1.050 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.889, 90.799, 83.934 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.100 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.26029 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.353 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.039 | 0.396 |
| Number of reflections | 40346 | |
| Completeness [%] | 96.8 | 96.8 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8000, zinc acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
