1Z1C
Structural Determinants of Tissue Tropism and In Vivo Pathogenicity for the Parvovirus Minute virus of Mice
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | FUJI |
Wavelength(s) | 0.91 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 448.700, 416.700, 305.300 |
Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.500 |
Rwork | 0.325 |
R-free | 0.32900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | canine parvovirus model |
RMSD bond length | 0.010 |
RMSD bond angle | 1.619 |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 3.500 |
Rmerge | 0.155 |
Number of reflections | 437558 |
Completeness [%] | 63.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 10mM Tris-HCl, 8 mM CaCl2, PEG8000, virus 10mg/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP |