1Z1B
Crystal structure of a lambda integrase dimer bound to a COC' core site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-13 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.9786 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 97.599, 211.278, 191.704 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.800 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.29600 |
Structure solution method | MIRAS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | TRUNCATE |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.780 |
High resolution limit [Å] | 3.650 | 3.650 |
Number of reflections | 22529 | |
Completeness [%] | 99.0 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | PEG4000, Sodium citrate, isopropanol, DTT, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG400 | ||
2 | 1 | 1 | Sodium citrate | ||
3 | 1 | 1 | isopropanol | ||
4 | 1 | 1 | DTT | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | PEG400 | ||
7 | 1 | 2 | Sodium citrate |