1Z0X
Crystal structure of transcriptional regulator, tetR Family from Enterococcus faecalis V583
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-20 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97945, 0.97959, 0.97882 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 88.418, 126.810, 38.391 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.222 |
Rwork | 0.219 |
R-free | 0.28300 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.168 | 0.682 |
Number of reflections | 17510 | |
<I/σ(I)> | 12.4 | 1.64 |
Completeness [%] | 99.2 | 93.6 |
Redundancy | 8.3 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | Ammonium sulfate, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |